effects of notch signaling  (Proimmune)

Journal: Signal Transduction and Targeted Therapy

Article Title: Notch signaling pathway: architecture, disease, and therapeutics

doi: 10.1038/s41392-022-00934-y

Figure Lengend Snippet: A brief history of the NOTCH signaling pathway. T-ALL, T cell acute lymphoblastic leukemia; AGS, Alagille syndrome; GSI, γ-secretase inhibitor

Article Snippet: Fourth, conditions triggering the anti-immune or proimmune effects of NOTCH signaling in tumor cells should be considered.

Techniques:

Journal: Signal Transduction and Targeted Therapy

Article Title: Notch signaling pathway: architecture, disease, and therapeutics

doi: 10.1038/s41392-022-00934-y

Figure Lengend Snippet: Overview of the NOTCH signaling pathway and therapeutic targets. In signal-receiving cells, NOTCH receptors are first generated in the ER and then trafficked to the Golgi apparatus. During trafficking, NOTCH receptors are glycosylated at the EGF-like repeat domain (red curves). Then, in the Golgi apparatus, NOTCH receptors are cleaved into heterodimers (S1 cleavage) and transported to the cell membrane. With the help of ubiquitin ligases, some of the NOTCH receptors on the cell membrane are endocytosed into endosomes. Endosomes contain an acidic environment with ADAMs and γ-secretase. The NOTCH receptors in endosomes can be recycled to the cell membrane, cleaved into NICD, or transported into lysosomes for degradation. In signal-sending cells, NOTCH ligands are distributed on the cell membrane and can bind to NOTCH receptors on signal-receiving cells. However, the ligands are inactive before ubiquitylation by Neur or Mib. After ubiquitylation, ligands can be endocytosed, thus producing a pulling force for the binding receptors. Without the pulling force, the S2 site (red marks) of NOTCH receptors is hidden by the NRR domain, and thus, the NOTCH receptors are resistant to cleavage by ADAMs. With the pulling force, the NRR domain is extended, therefore exposing the S2 site for cleavage. ADAMs and the pulling force are both necessary for S2 cleavage. After S2 cleavage, the remaining part of the NOTCH receptor is called NEXT. NEXT can be further cleaved on the cell membrane by γ-secretase or endocytosed into endosomes. In the former mode, NICD is released on the cell membrane. In the latter mode, NEXT can be cleaved into NICD or transported into lysosomes for degradation. In total, there are three approaches to generate NICD, classified as ligand-independent activation, ligand-dependent endocytosis-independent activation, and ligand-dependent endocytic activation. NICD can be translocated into the nucleus or remain in the cytoplasm to crosstalk with other signaling pathways, such as NFκB, mTORC2, AKT, and Wnt. The classical model proposes that, in the absence of NICD, CSL binds with corepressors to inhibit the transcription of target genes. Once NICD enters the nucleus, it can bind with CSL and recruit MAMLs, releasing corepressors, recruiting coactivators, and thus promoting the transcription of NOTCH target genes. There are two main approaches to inhibit NOTCH signaling for therapy. One is designing inhibitors of the key components of the pathways, including the enzymes that participate in S1 cleavage, ADAMs, γ-secretase, and MAML. The other one is producing antibody-drug conjugates against NOTCH receptors and ligands. The protein structures of NOTCH ligands and receptors are shown in the top left corner. NICD, NOTCH intracellular domain; ADAM, a disintegrin and metalloproteinase domain-containing protein; Neur, Neuralized; Mib, Mindbomb; NRR, negative regulatory region; NEXT, NOTCH extracellular truncation; CSL, CBF-1/suppressor of hairless/Lag1; MAMLs, Mastermind-like proteins; TM, transmembrane domain; RAM, RBPJ association module; ANK, ankyrin repeats; PEST, proline/glutamic acid/serine/threonine-rich motifs; NLS, nuclear localization sequence; CoR, corepressor; CoA, coactivator; ub, ubiquitin

Article Snippet: Fourth, conditions triggering the anti-immune or proimmune effects of NOTCH signaling in tumor cells should be considered.

Techniques: Biomarker Discovery, Generated, Membrane, Ubiquitin Proteomics, Binding Assay, Activation Assay, Protein-Protein interactions, Sequencing

Journal: Signal Transduction and Targeted Therapy

Article Title: Notch signaling pathway: architecture, disease, and therapeutics

doi: 10.1038/s41392-022-00934-y

Figure Lengend Snippet: The role of NOTCH signaling in body development and damage repair. NOTCH signaling is involved in regulating the differentiation and function of stem cells, affecting organ production and damage repair. a NOTCH signaling promotes the self-renewal of stem cells, induces multipotent progenitors for lineage selection, and generates different terminal cells; when the organ is damaged, cell type A is damaged and destroyed, and the stimulated cell type B rapidly upregulates the expression of NOTCH signaling to promote their own proliferation, and is partially redifferentiated into cell type A. b Highly activated NOTCH induces the expression of bile duct cell-enriched transcription factors and promotes the differentiation of multipotent hepatocyte progenitors into bile duct epithelial cells. c In liver injury, BEC are damaged and destroyed. NOTCH signaling is highly expressed in hepatocytes, which are further transformed into biphenotypic cells, which manifests the biliary tract morphology, and finally generate new BEC (BEC’) to form small tubular structures. HPC, hematopoietic progenitor cell; BEC, bile duct epithelial cell; SOX9, SRY-related high-mobility group box 9; HNF, hepatocyte nuclear factor

Article Snippet: Fourth, conditions triggering the anti-immune or proimmune effects of NOTCH signaling in tumor cells should be considered.

Techniques: Selection, Expressing, Transformation Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: Notch signaling pathway: architecture, disease, and therapeutics

doi: 10.1038/s41392-022-00934-y

Figure Lengend Snippet: NOTCH Signaling in Noncancerous diseases

Article Snippet: Fourth, conditions triggering the anti-immune or proimmune effects of NOTCH signaling in tumor cells should be considered.

Techniques: Expressing, Mutagenesis, Transduction, Activation Assay, Inhibition

Journal: Signal Transduction and Targeted Therapy

Article Title: Notch signaling pathway: architecture, disease, and therapeutics

doi: 10.1038/s41392-022-00934-y

Figure Lengend Snippet: NOTCH Signaling in Cancers

Article Snippet: Fourth, conditions triggering the anti-immune or proimmune effects of NOTCH signaling in tumor cells should be considered.

Techniques: Activation Assay, Expressing, In Vivo, In Vitro, Virus, Over Expression

Journal: Signal Transduction and Targeted Therapy

Article Title: Notch signaling pathway: architecture, disease, and therapeutics

doi: 10.1038/s41392-022-00934-y

Figure Lengend Snippet: NOTCH signaling pathway in antitumor immunity. NOTCH signaling plays important roles in both tumor-suppressive and tumor-promoting immune cells. NOTCH signaling promotes the differentiation of many immune cells. DLL and JAG mediate both similar and distinct effects. DC, dendritic cell; CD8T, CD8+ T cell; MDSC, myeloid-derived suppressor cell; CD4T, CD4+ T cell; Th1, type1 T helper cell; Th2, type2 T helper cell; Treg, regulatory T cell; TAM, tumor-associated macrophage; TAN, tumor-associated neutrophil; PD-1, programmed death-1; EOMES, eomesodermin; GZMB, granzyme B; DLL, delta-like ligand; CCL2, C-C motif chemokine ligand 2

Article Snippet: Fourth, conditions triggering the anti-immune or proimmune effects of NOTCH signaling in tumor cells should be considered.

Techniques: Derivative Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: Notch signaling pathway: architecture, disease, and therapeutics

doi: 10.1038/s41392-022-00934-y

Figure Lengend Snippet: Drugs targeting the NOTCH signaling pathway assessed in clinical trials

Article Snippet: Fourth, conditions triggering the anti-immune or proimmune effects of NOTCH signaling in tumor cells should be considered.

Techniques: Mutagenesis, Expressing, Inhibition, Produced, Activity Assay, Activation Assay